›› 2011, Vol. 23 ›› Issue (6): 456-459.doi: 10.3969/j.issn.1004-616x.2011.06.013

• 核辐射安全专家论坛 • Previous Articles     Next Articles

Effects of X-rays on the expression of Bmi-1 gene in human lung adenocarcinoma cell line A549

LIANG Xiao-fang，ZOU Yue，JIANG Qi-sheng，LI Feng-sheng，WANG Lu，ZHOU Xiang-yan   

  1. 1. Second Artillery General Hospital of PLA, Beijing 100088; 2. Suzhou University Medical College, Suzhou 215123，Jiangsu; 3. Fudan University Shanghai Cancer Center, Shanghai 200032; 4. National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2011-09-19 Revised:2011-09-21 Online:2011-11-30 Published:2011-11-30
  • Contact: ZOU Yue

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Abstract: OBJECTIVE: To study the dose and time effects of X-rays on the expressions of Bmi-1 gene and protein in human lung adenocarcinoma cell line A549. METHODS：A549 cell line was exposed to different doses of X-rays (2，4，6 Gy )and the relative level of Bmi-1 mRNA and protein was detected by using quantitative real-time PCR and Western blot at 6，12，24，48，72 h after irradiation. The untreated A549 cells were used as control. RESULTS： Bmi-1 mRNA in A549 cells was highly regulated after irradiated with 2，4，6 Gy and the peaked at 6 h，12 h and 24 h respectively. The expression of Bmi-1 mRNA，except for 2 Gy group，after 48 h showed statistical difference compared with the untreated group(P<0.05). After radiation， the expression of Bmi-1 protein was significant at most time points (P<0.05)，increased during 48 h，but was decreased after 48 h，and approached level of the untreated at 72 h. CONCLUSION：Under our experimental conditions，2-6 doses of X-rays radiation could increase the expressions of Bmi-1 mRNA and protein in A549 cells .

Key words: lung cancer, Bmi-1, A549 cells, X-rays